Urine Culture

LIGHT SCATTERING TECHNOLOGY APPLIED TO URINE CULTURE

Urinary Tract infections (UTI’s) are considered to be one of the most common human bacterial infections second only to respiratory infections. The incidence of UTI’s increases with the presence of diabetes, malformations of the urinary tract system as well as with age. UTI’s are also the most common nosocomial infections mostly linked to urethral catheters and invasive diagnostic procedures.

  • Alfred 60 and HB&L  automated systems are designed for urine culture and offer high sensitivity and specificity.
  • Using patented light scattering technology the Alfred 60 and HB&L are able to monitor bacteria replication activity from the inoculum into specific culture broths and provide real time growth curves.
  • Quantitative bacterial count results are reported in CFU/ml.
  • Strong positive samples are flagged after only 45 minutes of incubation. A positive threshold can be customized according to the sensitivity requirements of the laboratory and patient.
  • Three analytical protocols are available: Fast, Standard and Boric Acid setting.
  • A specific eugonic broth has been developed to guarantee optimized aerobic pathogen bacteria growth.
  • Broths are contained in sterile vials with pierceable hermetic seals, reducing contaminations.
  • Samples are incubated at 37°C and constantly mixed avoiding sedimentation, flotation and growth anomalies typical of several micro-organisms.
  • Only live bacteria are detected while interference from non replicating substances such as erythrocytes, leukocytes, dead cells and salts present in the sample are eliminated during the initial zero reading.

Learn more about ALIFAX ® Urine Culture applications here

Human Biological Liquids Culture

LIGHT SCATTERING TECHNOLOGY APPLIED TO BACTERIAL CULTURE

The rapid analysis of human biological liquids is important to patients for whom the timely diagnosis and beginning of adequate therapy can make a difference to their prognosis. In addition to community acquired infections, hospital acquired infections have noticeable impact on public health. Their increased morbidity and mortality rates as well as prolonged hospital stays represent additional diagnostic and treatment costs.

  • Alfred 60 and HB&L automated systems are designed for human biological liquids and offer high sensitivity and specificity.
  • Using patented light scattering technology the Alfred 60 and HB&L are able to monitor bacteria replication activity from the inoculum step into specific culture broths providing real time growth curves.
  • Quantitative bacterial count results are reported in CFU/ml.
  • Due to the optimization of the broth it is possible to perform the bacterial culture in sterile endocavitary samples such as respiratory fluids, cerebrospinal fluid and pleural fluid offering a sensitivity of 1 CFU/ml in 6 hours.
  • Enhanced liquid culture media combined with a specific supplement (DEB) has been developed to detect aerobic bacteria and exigent micro-organisms such as Haemophilus influenza, Neisseria meningitidis as well as samples characterized by extremely low bacterial counts.
  • Broths are contained in sterile vials with pierceable hermetic seals, thus considerably reducing contaminations.
  • Samples are incubated at 37°C and constantly mixed avoiding sedimentation, flotation and growth anomalies typical of several micro-organisms.
  • Only live bacteria are detected while interference from non replicating substances such as erythrocytes, leukocytes, dead cells and salts present in the sample are eliminated during the initial zero reading.

Learn more about ALIFAX® Human Biological Liquids Culture here

Antimicrobial Susceptibility Testing

LIGHT SCATTERING TECHNOLOGY APPLIED TO SUSCEPTIBILITY TESTING

In cases of serious bacterial infections the timely administration of an effective antibiotic therapy increases disease resolution and patient survival. For this reason, the microbiology laboratory has to provide “clinically useful results” in order to guide the clinician to choose the most appropriate antibiotic therapy as soon as possible. Rapid Antimicrobial Susceptibility Test (AST) results facilitate effective treatment, reduce the number of laboratory tests, days of hospitalization and public health costs. In addition, they are useful in monitoring the evolution of resistant bacteria.

Alfred 60 and HB&L  automated systems are designed to properly monitor the turbidity levels of the samples simultaneously to the culture test.
Positive culture samples can be flagged by the instruments when the turbidity reaches the 0.5 McFarland (by the McFarland Monitor).

Monomicrobial samples can then be tested with a customized antibiotic panel providing results within few hours. The results are expressed in percentage of resistance to the antibiotic and classified as Resistant, Intermediate or Sensitive.

Results are available within 3 hours after the start of analysis.

Learn more about ALIFAX® Antimicrobial Susceptibility Testing here

Residual Antimicrobial Activity

ALIFAX RESIDUAL ANTIMICROBIAL ACTIVITY TEST

The Alfred 60 and HB&L systems perform the RAA test and bacterial culture simultaneously. Using Staphylococcus epidermidis as a growth control it is possible to check for the presence of antibiotics and drugs or foods with antibacterial properties that inhibit bacterial growth in a sample. Bacterial culture the native sample is inoculated at the same time into a vial containing a Staphylococcus epidermidis suspension which is sensitive to most of the commonly used antibiotics. The systems allow the determination of Residual Antibiotic Activity in the sample by comparing the Staphylococcus epidermidis with the bacterial culture growth curves. The Alifax RAA test is also useful to monitor the effectiveness of therapy and verify if the antibiotic has reached the infection site. RAA test results are simultaneously displayed with bacterial growth results in Urine and Human Biological Liquid cultures.

Learn more about ALIFAX® Residual Antimicrobial Activity here

Contact us at 780 702 9500 or complete the form.